| Microorganism name |
Komagatella phaffi (Pichia pastoris)
|
| Target proteins |
Brazzein (Rong et al., 2025)
|
| Wild-type or GMO |
GMO (Rong et al., 2025)
|
| Production mode (intracellular/extracellular) |
Extracellular (Rong et al., 2025)
|
| Protein yield (g/L or g/g?) |
0.639 g/L (Rong et al., 2025)
|
| Temperature used in study |
30°C (Rong et al., 2025)
|
| pH used in study |
pH 5.5 (Rong et al., 2025)
|
| C & N source |
Glucose, yeast extract, peptone (YPD medium) & glycerol (Rong et al., 2025)
|
| Regulatory status in Europe |
Not allowed |
| Regulatory status in other parts of the world |
FDA GRAS approval in the US. Not allowed in Canada
|
| Companies |
Perfect Day has recieved GRAS notice for the production of brazzein in K. phaffi
|
| Publications/references |
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Rong, W., Wang, X., Shi, Z., Liu, L., Zhang, Z., Fang, Y., Sun, J., Lin, L., & Wang, G. (2025). Systematic Engineering of Komagataella phaffii for Efficient Production of Sweet‐Tasting Protein Brazzein. Biotechnology Journal, 20(6). https://doi.org/10.1002/biot.70046
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Spohner, S. C., Müller, H., Quitmann, H., & Czermak, P. (2015). Expression of enzymes for the usage in food and feed industry with Pichia pastoris. Journal of Biotechnology, 202, 118–134. https://doi.org/10.1016/j.jbiotec.2015.01.027
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Lv, W., & Cai, M. (2025). Advancing Recombinant Protein Expression in Komagataella phaffii: Opportunities and Challenges. FEMS Yeast Research. https://doi.org/10.1093/femsyr/foaf010
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Pan, Y., Yang, J., Wu, J., Yang, L., & Fang, H. (2022). Current advances of Pichia pastoris as cell factories for production of recombinant proteins. Frontiers in Microbiology, 13. https://doi.org/10.3389/fmicb.2022.1059777
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Barone, G. D., Emmerstorfer-Augustin, A., Biundo, A., Pisano, I., Coccetti, P., Mapelli, V., & Camattari, A. (2023). Industrial Production of Proteins with Pichia pastoris—Komagataella phaffii. Biomolecules, 13(3), 441. https://doi.org/10.3390/biom13030441
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Ergün, B. G., Laçın, K., Çaloğlu, B., & Binay, B. (2022). Second generation Pichia pastoris strain and bioprocess designs. Biotechnology for Biofuels and Bioproducts, 15(1). https://doi.org/10.1186/s13068-022-02234-7
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